Soil-transmitted helminth infection is considered one of the Neglected Tropical Diseases (NTDs) worldwide. The Philippines is one of the countries in Asia with the highest prevalence of parasite infection. Adding to this problem is the resistance of pathogenic nematodes to commercial drugs in livestock hosts. Capsicum species exhibit antiparasitic
activity against a wide range of endoparasites as fed to chicken. This study assessed the anthelmintic activity of the Capsicum annuum var. longum against gastrointestinal parasites found in broiler chicken stool and to identify the secondary metabolites from the placental region of the chili’s fruit. Phytochemical screening test indicated the presence of alkaloid, flavonoid, and tannins. The ethanolic extracts of 1%, 10%, 50%, and 100% concentrations were administered to a total of 15 broiler chickens. The anthelmintic efficacy of the plant extract was evaluated through Fecal Egg Count Method. All treatments displayed very high effectiveness on reducing egg parasite count, which was comparable to the positive control. The result indicated that the Capsicum annuum var. longum (Siling-Haba) extracts were very effective against gastrointestinal parasites of the broiler chicken. This potency may be attributed to the high level of capsaicinoid content in the fruit. The findings may lead to a new organic anthelmintic drug alternative that is also environmentally sustainable.

1. 1. 1. Z. A. Silver, S. P. Kaliappan, P. Samuel, S. Venugopal, G. Kang, R. Sarkar, and S. S. Ajjampur, “Geographical distribution of soil transmitted helminths and the effects of community type in South Asia and South East Asia: A systematic review,” PLoS Neglected Tropical Diseases, vol. 12, no. 1, pp. 145– 153, 2018. doi: https://doi.org/10.1371/journal.pntd.0006153
      2. V. Alviar, “Philippines one of asia’s ‘wormiest’ countries,” 2013. [Online]. Available: https://bit.ly/34snCom
      3. World Health Organization, “Deworming: Every girl and every woman has the right to be treated,” 2017. [Online]. Available: https://bit.ly/3nmBbhH
      4. Centers for Disease Control and Prevention, “Neglected tropical diseases. global health–division of parasitic diseases and malaria,” 2017. [Online]. Available: https://bit.ly/34xUf3O
      5. R. M. Kaplan, “Drug resistance in nematodes of veterinary importance: A status report,” Trends in Parasitology, vol. 20, no. 0, pp. 477–481, 2004. doi: https://doi.org/10.1016/j.pt.2004.08.001
      6. L. L. Mortensen, L. H. Williamson, T. H. Terrill, R. A. Kircher, M. Larsen, and R. M. Kaplan, “Evaluation of prevalence and clinical implications of anthelmintic resistance in gastrointestinal nematodes in goats,” Journal of the American Veterinary Medical Association, vol. 223, no. 4, pp. 495–500, 2003. doi: https://doi.org/10.2460/javma.2003.223.495
      7. R. Herren, The Science of Animal Agriculture. NewYork, NY: Delmar Publisher International Thomson Publishing Company, 2012.
      8. Department of Health, “Traditional & alternative medicine,” 1997. [Online]. Available: https://bit.ly/2Sw48tf
      9. A. E. Al-Snafi, “The pharmacological importance of capsicum species (Capsicum annuum and Capsicum frutescens) grown in Iraq,” Journal of Pharmaceutical Biology, vol. 5, no. 3, pp. 124–142, 2015.
      10. M. G. Frial McBride, “Extraction of resins from capsicum annuum var longum (siling haba) for the study of their potential anti-microbial activities,” Journal
          of Chemical and Pharmaceutical Research, vol. 8, no. 3, pp. 117–127, 2016.
      11. A. G. Namdeo, “Plant cell elicitation for production of secondary metabolites: A review,” Pharmacogn Revolution, vol. 1, no. 1, pp. 69–79, 2007. doi: https://doi.org/10.1111/j.1751-7915.2010.00196.x
      12. A. A. El-Deek, M. A. Al-Harthi, M. Osman, F. AlJassas, and R. Nassar, “Hot pepper (capsicum annum) as an alternative to oxytetracycline in broiler diets and effects on productive traits, meat quality, immunological responses and plasma lipids,” European Polity Science, vol. 76, no. 2, pp. 73–80, 2012. doi: https://doi.org/10.1017/s1751731110000820
      13. X. Dong, X. Li, L. Ding, F. Cui, Z. Tang, and Z. Liu, “Stage extraction of capsaicinoids and red pigments from fresh red pepper (capsicum) fruits with ethanol as solvent,” Food Science and Technology, vol. 59, no. 1, pp. 396 402, 2014. doi: https://doi.org/10.1016/j.lwt.2014.04.051
      14. R. M. Stoica, M. Moscovici, C. Tomulescu, and N. Babeanu, “Extraction and analytical methods of capsaicinoids-a review,” Scientific Bulletin Biotechnologies, vol. 20, no. 5, pp. 93–98, 2016.
      15. M. S. Chinn, R. R. Sharma-Shivappa, and J. L. Cotter, “Solvent extraction and quantification of capsaicinoids from capsicum chinense,” Food and Bioproducts Processing, vol. 89, no. 4, pp. 340– 345, 2011. doi: https://doi.org/10.1016/j.fbp.2010.08.003
      16. P. Tiwari, B. Kumar, M. Kaur, G. Kaur, and H. Kaur, “Phytochemical screening and extraction: a review,” Internationale Pharmaceutica Sciencia, vol. 1, no. 1, pp. 98–106, 2011.
      17. S. N. Deshpande, “Preliminary phytochemical analysis and in vitro investigation of antibacterial activity of acacia nilotica against clinical isolates,” Journal of Pharmacognosy and Phytochemistry, vol. 1, no. 5, pp. 56–80, 2013.
      18. G. Parinas, M. Alboir, B. Panelo, R. Nicolas, and P. Agsalud, “Ampalaya (momordica charantia) leaf extract against gastro-intestinal parasites of native chicken,” Asia Pacific Journal of Multidisciplinary Research, vol. 5, no. 2, pp. 212–218, 2017.
      19. A. Roepstorff and P. Nansen, Epidemiology, Diagnosis and Control of Helminth Parasites of Swine. Rome, Italy: Food Organization, 1998.
      20. M. Morgenstern, A. Nies, M. Goecke, and R. Hanewinkel, “E-cigarettes and the use of conventional cigarettes: A cohort study in 10th grade students in Germany,” Deutsches Ärzteblatt International, vol. 115, no. 14, pp. 243–256, 2018. doi: https://doi.org/10.3238/arztebl.2018.0243
      21. M. W. Dong, “Instruments & applications how hot is that pepper? quantifying capsaicinoids with chromatography,” Todays Chemist at Work, vol. 9, no. 5, pp. 17–22, 2000.

M. C. Gentiles, M. G. D. Rollo, and N. E. Morales, “Anthelmintic activity of capsicum annuum var. longum (siling-haba) placental extracts against gastrointestinal parasites in broiler chicken stool,” International Journal of Applied and Physical Sciences, vol. 5, no. 2, pp. 58-63, 2019. doi: https://dx.doi.org/10.20469/ijaps.5.50004-2