The study’s objectives were to develop a methodology for the isolation of soil nano-fraction (colloid-liquid phase) and determine the content of elements and study its distribution in the liquid phase and the soil samples. Nanofraction (colloid-salt fraction) is the part of a sample with a particle size less than 1000 nm. Nanofraction (NF) was isolated with deionized hot water from soil samples 30 g (soil: water – 1:10). The suspension was mixed for 6 h, settled for 24 h, and filtrated using a membrane filter (<1 μm). Mass spectrometry with inductively coupled plasma (75 elements) analyzed the soil sample and its Nano-Fraction. The chemical characteristics of the extracted liquid phase of the soil reflect its water-soluble component properties. Comparing the data obtained with the analysis results for the whole soil allows the assessment of a share of easily mobile and difficultly mobile forms of chemical compounds. This is the necessary basis for the development of a model describing the transport of metals in soil.

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