This study aims to determine the effect of artificial feed made from aquatic weeds (Pistia sp., Salvinia sp., and Lemna sp.) through fermentation of Rhyzopus oryzae on the growth of Climbing Perch fish. The experimental factors were aquatic weeds, namely: Pistia sp. (Kayu apu), Salvinia sp. (Kiambang), and Lemna sp. (Gulma itik) as Factor A and different percentages of Rhyzopus oryzae fermentation (5%, 10%, and 15%) as Factor B. Each treatment was conducted in three replications. The results showed that the combination of Salvinia sp. in 15% fermentation provided the best absolute growth of 2.31g, the best relative growth of 416.04%, the best feed conversion of 2.00, the highest feed efficiency of 50.0%, and the highest protein retention of 13.79%. The results of the ANOVA test of the feeding trials showed no significant differences among treatment combinations with regards to absolute growth, relative growth, feed conversion, feed efficiency, and protein and fat retention. The experimental Climbing Perch fish had a survival rate of 100%. Water quality data, such as temperature, DO, pH, and NH3, showed that the living conditions are tolerable for the Climbing Perch fish.

1. A. M. Halim, M. Nabi, and S. Nahar, “Study on optimization of stocking density of climbing perch (Anabas testudineus, bloch 1792) in marginal farmer earthen ponds,” Journal of Entomology and Zoology Studies, vol. 5, no. 3, pp. 833–837, 2017.
2. E. Afrianto and E. Liviawaty, Fish Feed Making, Storage, Testing, Development. Yogyakarta, Indonesia: Penerbit Kanisius, 2005.
   
3. B. Ghosh and R. R. Ray, “Current commercial perspective of rhizopus oryzae: A review,” Journal of Applied Science, vol. 11, no. 14, pp. 2470–2486, 2011. doi: https://doi.org/10.3923/jas.2011.2470.2486
   
4. D. Sanjayasari et al., “Estimation of nisbah proteins of sold fish food (mystus nigriceps) nutrition basis for domesticity success,” Journal of Fisheries and Marine, vol. 15, no. 02, pp. 89–97, 2012.
   
5. I. Torang, “Growth of the fish seed (Anabas testudineus bloch) with additional feeding of maggot,”Journal of Tropical Animal Science, vol. 2, no. 1, pp. 12–16, 2013.
6. A. Mudjiman, Fish Food. Jakarta, Indonesia: Penebar Swadaya, 2004.
   
7. J. E. Setiawati, Y. T. Adiputra, S. Hudaidah et al., “The effect of adding probiotics to feed with different doses of growth, survival, feed efficiency and protein retention of catfish (pangasius hypophthalmus),” e-Journal of Aquaculture Engineering and Technology, vol. 1, no. 2, pp. 151–162, 2013.
8. J. Akbar, Fish Betok Cultivation and Business Opportunity. Yogyakarta, Indonesia: EJA Publisher, 2012.
   
9. J. Akbar, A. Mangalik, and S. Fran, “Application of fermented aquatic weeds in formulated diet of climbing perch (Anabas testudineus),” International Journal of Engineering Research & Science (IJOER), vol. 2, pp. 240–243, 2016.
10. H. K. Susanto, Cultivation of Patin Fish. Jakarta, Indonesia: Penebar Swadaya, 2000.
    
11. J. Y. Loh and A. S. Y. Ting, “Comparative study of analogue hormones and the embryonic, larval and juvenile development on the induced breeding of climbing perch (Anabas testudineus, bloch, 1792),” International Journal of Fisheries and Aquatic Studies, vol. 2, pp. 277–278, 2015. 

Elrifadah, R. Iskandar and H. Fitrah, “Artificial feed production from fermented aquatic weeds and its implementation in climbing perch fish (Anabas Testudineus),” International Journal of Applied and Physical Sciences, vol. 4, no. 1, pp. 1-5, 2018. doi: https://dx.doi.org/10.20469/ijaps.4.50001-1