Because of their nutritive value, milk and milk-made products are considered a very important food for all consumers, especially young ones. Consumers are at risk because of toxic chemicals and food-borne pathogens in milk, infants, young children, or persons with HIV/AIDS, cancer, diabetes, kidney disease, and transplant patients at greater risk. In the present study for milk-borne pathogens’ identification, DNA of bacterial isolates was amplified using a universal set of forwarding primer pA 5’AGA GTT TGA TCC TGG CTC AG 3’ (828) and reverse primer pH 5’AAG GAG GTG ATC CAG CCG CA 3’( 1542 1522), corresponding to positions 828 and 15421522, respectively from Escherichia coli. 16s rRNA gene sequences were amplified and sequenced. Twelve microorganisms are Salmonella enterica, Citrobacter koseri, Escherichia fergusonii, Klebsiella pneumonia, Serratia liquifaciens, Shigella flexneri, Kosakonia sacchari and Enterobacter = species, Klebsiella vericola, Escherichia vulneris, and Escherichia coli strains based on 16s rRNA sequences Submission to NCBI Gene Bank. Some of them have shown pathogenecity and resistance to some antibiotics, which should be properly identified for risk analysis to consumers. In our previous study, we have also reported the presence of carcinogens, phthalates, and hormone disrupters in milk. Food adulterants, chemicals, and pathogens’ presence are major causes for developing the disorder in children that needs scientific community attention.

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