The Survival of Endophytic Bacteria Isolated from Jerusalem Artichoke In Drought Conditions
Volume 4, Issue 2 J. Namwongsa, S. Boonlue, N. Riddech, S. Jogloy, W. Mongkolthanaruk
Published online:13 July 2018
Article Views: 35
Abstract
Four endophytic bacteria isolated from Jerusalem artichoke had the ability of plant growth promotion. These bacteria, isolate 3.13, 4.43, 5.2, and 5.18, were selected to study the promotion of plant growth in drought conditions. Thus, the survival of the bacteria under drought conditions needed for the investigation. All four isolates showed Nutrient Broth (NB) growth containing 20% Polyethylene Glycol (PEG). Moreover, all four isolates were tested for their survival in NB with 20% PEG and soil by incubating at 30, 35, 40, and 45◦C. Reasonable survival was observed in all isolates at 30 to 40◦C of both media. Furthermore, isolate 5.18 demonstrated the growth at 45◦C and maintained cell numbers at 4.19×104 CFU/mL after 29 days and 1.30×107 CFU/mL after 57 days in NB with 20% PEG and in the soil, respectively.
Reference
C. Lesk, P. Rowhani, and N. Ramankutty, “Influence of extreme weather disasters on global crop production,” Nature, vol. 529, no. 7584, pp. 84–87,2016. doi: https://doi.org/10.1038/nature16467J.
Conde, J. Tenorio, B. Rodriguez-Maribona, and L. Ayerbet, “Tuber yield of jerusalem artichoke (Helianthus tuberosus L.) in relation to wa-ter stress,” Biomass and Bioenergy, vol. 1, no. 3, pp. 137–142, 1991. doi: https://doi.org/10.1016/0961-9534(91)90022-5
A. Monti, M. Amaducci, and G. Venturi, “Growth response, leaf gas exchange and fructans accumula-tion of Jerusalem artichoke (Helianthus tuberosus L.) as affected by different water regimes,” European Journal of Agronomy, vol. 23, no. 2, pp. 136–145, 2005. doi: https://doi.org/10.1016/j.eja.2004.11.001
B. Azis, B. Chin, M. Deacon, S. Harding, and G. Pavlov, “Size and shape of inulin in dimethyl sulphoxide solution,” Carbohydrate Polymers, vol. 38, no. 3, pp. 231–234, 1999. doi: https://doi.org/10.1016/s0144-8617(98)00096-4
J. G. Muir, S. J. Shepherd, O. Rosella, R. Rose, J. S. Barrett, and P. R. Gibson, “Fructan and free fructose content of common Australian vegetables and fruit,” Journal of Agricultural and Food Chemistry, vol. 55, no. 16, pp. 6619–6627, 2007. doi: https://doi.org/10.1021/jf070623x
L. Yang, Q. S. He, K. Corscadden, and C. C. Udenigwe, “The prospects of jerusalem artichoke in functional food ingredients and bioenergy production,” Biotechnology Reports, vol. 5, pp. 77–88, 2015. doi: https://doi.org/10.1016/j.btre.2014.12.004
X. Y. Ma, L. H. Zhang, H. B. Shao, G. Xu, F. Zhang, F. T. Ni, and M. Brestic, “Jerusalem artichoke (Helianthus tuberosus), a medicinal salt- resistant plant has high adaptability and multiple-use values,” Journal of Medicinal Plants Research, vol. 5, no. 8, pp. 1272–1279, 2011.
N. Vorasoot and S. Jogloy, “Inulin: Non-digestable carbohydrate as soluable fiber from Kaentawan for human health,” Kaen Kaset, vol. 34, no. 85-91, Feb. 2006.
S. Jogloy, V. Ladbuakaew, and R. Mikeaw, “Kaentawan (Helianthus tuberosus L.): A new energy crop,” Khon Kaen Agricultural Journal, vol. 3, no. 2, pp. 104–111, 2006.
K. Sritongon, “Screening of plant growth promoting rhizobacteria from rhizosphere soil for improving growth of Jerusalem artichoke,” Master thesis, Graduate School, Khon Kaen University, Khon Kaen, Thailand, 2015.
S. Compant, B. Mitter, J. G. Colli-Mull, H. Gangl, and A. Sessitsch, “Endophytes of grapevine flowers, berries, and seeds: Identification of cultivable bacteria, comparison with other plant parts, and visualization of niches of colonization,” Microbial Ecology, vol. 62, no. 1, pp. 188–197, 2011.
F. Chi, S.-H. Shen, H.-P. Cheng, Y.-X. Jing, Y. G. Yanni, and F. B. Dazzo, “Ascending migration of endophytic rhizobia, from roots to leaves, inside rice plants and assessment of benefits to rice growth physiology,” Applied and Environmental Microbiology, vol. 71, no. 11, pp. 7271–7278, 2005. doi: https://doi.org/10.1128/aem.71.11.7271-7278.2005
P. Bunruk, D. Kantachote, and A. Sukhoom, “Isolation and selection of purple non-sulfur bacteria for phosphate removal in rearing water from shrimp cultivation,” Journal of Applied and Physical Sciences, vol. 3, no. 2, pp. 73–80, 2017. doi: https://doi.org/10.20474/japs-3.2.5
H. Mohebalian and S. Alizadeh, “The antimicrobial activity of green tea extract against the major food born bacteria,” Journal of Advances in Health and Medical Sciences, vol. 2, no. 2, pp. 54–60, 2016. doi: https://doi.org/10.20474/jahms-2.2.2
X. Liu, X. Li, Y. Li, R. Li, and Z. Xie, “Plant growth promotion properties of bacterial strains isolated from the rhizosphere of the Jerusalem arti-choke (Helianthus tuberosus L.) adapted to saline–alkaline soils and their effect on wheat growth,” Canadian Journal of Microbiology, vol. 63, no. 3, pp. 228–237, 2016. doi: https://doi.org/10.1139/cjm-2016-0511
A. Pavlo, O. Leonid, Z. Iryna, K. Natalia, and P. A. Maria, “Endophytic bacteria enhancing growth and disease resistance of potato (Solanum tuberosum L.),” Biological Control, vol. 56, no. 1, pp. 43–49, 2011. doi: https://doi.org/10.1016/j.biocontrol.2010.09.014
S. Harish, M. Kavino, N. Kumar, D. Saravanakumar, K. Soorianathasundaram, and R. Samiyappan, “Biohardening with plant growth promoting rhizosphere and endophytic bacteria induces systemic resistance against banana bunchy top virus,” Applied Soil Ecology, vol. 39, no. 2, pp. 187–200, 2008. doi: https://doi.org/10.17660/actahortic.2009.828.30
J. Shi, A. Liu, X. Li, S. Feng, and W. Chen, “Inhibitory mechanisms induced by the endophytic bacterium MGY2 in controlling anthracnose of papaya,” Biological Control, vol. 56, no. 1, pp. 2–8, 2011. doi: https://doi.org/10.1016/j.biocontrol.2010.09.012
P. Holliday et al., A dictionary of plant pathology. Cambridge, UK: Cambridge University Press, 1998.
B. Schulz and C. Boyle, “What are endophytes?” in Microbial root endophytes, B. J. E. Schulz, C. J. C. Boyle, and T. N. Sieber, Eds. Berlin, Germany: Springer, 2006, pp. 261–279.
P. Phetcharat and A. Duangpaeng, “Screening of endophytic bacteria from organic rice tissue for indole acetic acid production,” Procedia Engineering, vol. 32, pp. 177–183, 2012. doi: https://doi.org/10.1016/j.proeng.2012.01.1254
A. Sziderics, F. Rasche, F. Trognitz, A. Sessitsch, and E. Wilhelm, “Bacterial endophytes contribute to abiotic stress adaptation in pepper plants (Capsicum annuum L.),” Canadian Journal of Microbiology, vol. 53, no. 11, pp. 1195–1202, 2007. doi: https://doi.org/10.1139/w07-082
S. Z. Ali, V. Sandhya, and L. V. Rao, “Isolation and characterization of drought-tolerant ACC deaminase and exopolysaccharide-producing fluorescent pseudomonas sp.” Annals of Microbiology, vol. 64, no. 2, pp. 493–502, 2014. doi: https://doi.org/10.1007/s13213-013-0680-3
A. Elmi and C. West, “Endophyte infection effects on stomatal conductance, osmotic adjustment and drought recovery of tall fescue,” New Phytologist, vol. 131, no. 1, pp. 61–67, 1995. doi: https://doi.org/10.1111/j.1469-8137.1995.tb03055.x
C. Dimkpa, T. Weinand, and F. Asch, “Plant–rhizobacteria interactions alleviate abiotic stress conditions,” Plant, Cell & Environment, vol. 32, no. 12, pp. 1682–1694, 2009. doi: https://doi.org/10.1111/j.1365-3040.2009.02028.x
S. Mantelin and B. Touraine, “Plant growth-promoting bacteria and nitrate availability: Impacts on root development and nitrate uptake,” Journal of Experimental Botany, vol. 55, no. 394, pp. 27–34, 2004. doi: https://doi.org/10.1093/jxb/erh010
M. Caron, C. Patten, S. Ghosh, and B. Glick, “Effects of the plant growth promoting rhizobacterium pseudomonas putida gr12-2 on the physiology of canola roots,” in Plant Growth Regulation Society 22nd Conference, vol. 22, Minneapolis, MN, 1995, pp. 297–302.
C. M. Creus, R. J. Sueldo, and C. A. Barassi, “Water relations in Azospirillum-inoculated wheat seedlings under osmotic stress,” Canadian Journal of Botany, vol. 76, no. 2, pp. 238–244, 1998. doi: https://doi.org/10.1139/b97-178
B. R. Glick, D. M. Penrose, and J. Li, “A model for the lowering of plant ethylene concentrations by plant growth-promoting bacteria,” Journal of Theoretical Biology, vol. 190, no. 1, pp. 63–68, 1998. doi: https://doi.org/10.1006/jtbi.1997.0532
S. Mayak, T. Tirosh, and B. R. Glick, “Plant growth-promoting bacteria confer resistance in tomato plants to salt stress,” Plant Physiology and Biochemistry, vol. 42, no. 6, pp. 565–572, 2004. doi: https://doi.org/10.1016/j.plaphy.2004.05.009
H. B. Bal, L. Nayak, S. Das, and T. K. Adhya, “Isolation of ACC deaminase producing pgpr from rice rhizosphere and evaluating their plant growth promoting activity under salt stress,” Plant and Soil, vol. 366, no. 1-2, pp. 93–105, 2013. doi: https://doi.org/10.1007/s11104-012-1402-5
S. Konnova, O. Brykova, O. Sachkova, I. Egorenkova, and V. Ignatov, “Protective role of the polysaccharide-containing capsular components of azospirillum brasilense,” Microbiology, vol. 70, no. 4, pp. 436–440, 2001.
S. Sutton, “Determination of inoculum for microbiological testing,” Journal of GXP Compliance, vol. 15, no. 3, p. 49, 2011.
K. Sritongon, W. Mongkolthanaruk, S. Boonlue, S. Jogloy, D. Puangbut, and N. Riddech, “Rhi-zobacterial candidates isolated from Jerusalem artichoke (Helianthus tuberosus L.) rhizosphere for host plant growth promotion,” Chiang Mai Journal of Science, vol. 44, no. 1, pp. 83–93, 2017.
M. A. Gururani, C. P. Upadhyaya, V. Baskar, J. Venkatesh, A. Nookaraju, and S. W. Park, “Plant growth-promoting rhizobacteria enhance abiotic stress tolerance in Solanum tuberosum through inducing changes in the expression of ROS- scavenging enzymes and improved photosynthetic performance,” Journal of Plant Growth Regulation, vol. 32, no. 2, pp. 245–258, 2013. doi: https://doi.org/10.1007/s00344-012-9292-6
E. Armada, R. Azcón, O. M. López-Castillo, M. Calvo-Polanco, and J. M. Ruiz-Lozano, “Autochthonous arbuscular mycorrhizal fungi and bacillus thuringiensis from a degraded Mediterranean area can be used to improve physiological traits and performance of a plant of agronomic interest under drought conditions,” Plant Physiology and Biochemistry, vol. 90, pp. 64–74, 2015. doi: https://doi.org/10.1016/j.plaphy.2015.03.004
A. Marulanda, J.-M. Barea, and R. Azcón, “Stimulation of plant growth and drought tolerance by native microorganisms (am fungi and bacteria) from dry environments: Mechanisms related to bacterial effectiveness,” Journal of Plant Growth Regulation, vol. 28, no. 2, pp. 115–124, 2009.
